Drug Generic Names

Human Chemokine C Ligand 2 (CCL2)ELISA Kit

Purpose

Our Human Chemokine C Ligand 2 (CCL2)ELISA Kit is to assay CCL2 levels in serum, plasma, culture media or any biological fluid

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to CCL2 Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CCL2 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CCL2 and HRP conjugated CCL2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CCL2. You can calculate the concentration of CCL2 in the samples by comparing the OD of the samples to the standard curve.