|
Human CD40 ELISA Kit- Details |
|
Description |
Sandwich ELISA for quantitative detection of Human CD40 concentrations |
|
Applications |
SE |
|
Specificity |
1 pg/ml |
|
Gene Symbol |
CD40 |
|
Alternative Names |
TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40 |
|
Entrez Gene (Human) |
958 |
|
SwissProt (Human) |
P25942 |
|
Detection Method |
Colorimetric |
|
Sample Types |
Cell Culture Supernates, Serum, Plasma, Urine |
|
Test Principle |
|
The Cohesion Bioscience Human CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm |
Reviews
There are no reviews yet.