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Human Capn3 (Calcium-activated Neutral Proteinase 3) Elisa Kit |
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Sensitivity |
0.094ng/ml |
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Range |
0.156-10ng/ml |
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Alternative Name |
Calpain-3 ELISA Kit, Calcium-activated neutral proteinase 3 ELISA Kit, CANP 3 ELISA Kit, Calpain L3 ELISA Kit, Calpain p94 ELISA Kit, Muscle-specific calcium-activated neutral protease 3 ELISA Kit, New calpain 1 ELISA Kit, nCL-1 ELISA Kit, CAPN3 ELISA Kit, CANP3 ELISA Kit, CANPL3 ELISA Kit, NCL1 ELISA Kit |
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UniProt ID |
P20807 |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Specificity |
Specifically binds with CAPN3 , no obvious cross reaction with other analogues. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CAPN3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CAPN3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CAPN3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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