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Human Anti-NPHS1 (Nephrin) antibody ELISA Kit |
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Sensitivity |
4.688ng/ml |
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Range |
7.813-500ng/ml |
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Uniprot ID |
O60500 |
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Alternative Names |
Nephrin antibody ELISA Kit, Renal glomerulus-specific cell adhesion receptor antibody ELISA Kit, NPHS1 antibody ELISA Kit, NPHN antibody ELISA Kit |
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Detection Method |
Sandwich ELISA, Double Antigen |
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Detection Wavelength |
OD450 |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Reaction Time |
4H |
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Specificity |
Specifically binds with Anti-NPHS1 , no obvious cross reaction with other analogues. |
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Principle |
This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with Anti-NPHS1 bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-NPHS1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
