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Human Adiponectin ELISA Kit |
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Sensitivity |
15 pg/ml |
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Range |
62.5 pg/ml - 4000 pg/ml |
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Reactivity |
Human |
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Applications |
Sandwich ELISA |
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Detection Method |
Colorimetric |
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Sample Types |
Cell Culture Supernates, Serum, Plasma, Tissue Homogenates |
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SwissProt (Human) |
Q15848 |
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Entrez Gene (Human) |
9370 |
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Alternative Names |
CDC; ACRP30; APM1; GBP28; Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein |
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Description |
Sandwich ELISA for quantitative detection of Human Adiponectin concentrations |
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Storage |
Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles |
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Principle |
The Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. |
