Standard Curve
Storage2-8 â for 6 monthsRecovery
Matrices listed below were spiked with certain level of ACSL4/FACL4 and the recovery rates were calculated by comparing the measured value to the expected amount of ACSL4/FACL4 in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 90-98 | 92 |
| EDTA plasma(n=5) | 86-103 | 94 |
| heparin plasma(n=5) | 85-99 | 91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACSL4/FACL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=5) | 89-101% | 88-102% | 85-98% |
| EDTA plasma(n=5) | 82-97% | 86-101% | 87-98% |
| heparin plasma(n=5) | 80-99% | 80-100% | 80-100% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
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