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Human 15-epi-Lipoxin A4 (15-epi-LXA4) ELISA Kit- Details |
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Specificity |
No cross-reaction with other factors |
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Samples |
Human serum, plasma or cell culture supernatant |
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Assay Type |
Quantitative Sandwich |
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Intra-assay Precision |
<= 8% |
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Inter-assay Precision |
<= 12% |
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Preparation and Storage |
Store all reagents at 2-8 degree C. |
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Research Use |
For Research Use Only. Not for use in diagnostic procedures. |
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Test Principle |
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This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human 15-epi-LXA4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated. |
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