Description
| Product Name | HistoneH2A.X(Phospho-Ser139) Antibody |
| Catalogue No. | A6017-E11 |
| Gene Name | H2AX |
| Alternative Names | H2a/x |
| Swiss Prot ID | P16104 |
| All Sites: | Human: Ser139; Mouse: Ser139 |
| Form of Antibody: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability: | Store at -20℃/1 year |
| Reactivity | H,M,R |
| Application | WB,IHC,ELISA |
| Dilution | WB: 1:500~1:1000 ELISA: 1:10000 |
| Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E). |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
| Specificity/Sensitivity: | Histone H2A.X (Phospho-Ser139) antibody detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139. |
| References: | Alpna Tyagi, et al. (2005) Carcinogenesis; 26: 1978-1987.
James P, et al. (2002) Nucleic Acids Res; 30: 3698-3705. Mariana Yaneva, et al. (2005) Nucleic Acids Res; 33: 5320-5330. Jérôme Govin, et al. (2004) Eur. J. Biochem; 271: 3459-3469 |
| Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Histone H2A.X (Ab-139) antibody (Line 1 and 2) and Histone H2A.X (Phospho-Ser139) antibody (cat#:E11-7106A, Line 3 and 4).
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