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Histone H3 Recombinant Rabbit Monoclonal Antibody

Histone H3 Recombinant Rabbit Monoclonal Antibody

Catalogue No BSAB-297P
Host Rabbit
Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,FC
Size 20μL | 50μL | 100μL
Price POR
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SKU: BSAB-297P Categories: ,
Histone H3 Recombinant Rabbit Monoclonal Antibody
Description Recombinant Rabbit Monoclonal Antibody
Conjugation Unconjugated
Modification Unmodified
Isotype IgG
Clonality Monoclonal
Form Liquid
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% protective protein. Stable for 12 months from date of receipt.
Purification Affinity Purification
Gene Name Histone H3
Gene ID 8350
SwissProt ID P68431
Alternative Names H3/A; H3FA
Dilution Ratio WB 1:500-1:2000,IHC 1:2000-1:10000,ICC/IF 1:500-1:2000,FC 1:50-1:100
Molecular Weight Calculated MW:15 kDa; Observed MW:17 kDa
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.
Validated Image

Western blot analysis of extracts from Hela , 3T3 cells using Histone H3 Recombinant Rabbit Monoclonal Antibody at 1:1000

Immunofluorescence analysis of A431 cells labelling Histone H3 with AMRe86363