Scientific Background

High sensitive TMB Substrate Solution is widely used chromogenic substrate for Peroxidase (HRP) based detection system in ELISA. The reagent is clear to light yellow Solution. This is used for Endpoint assay using 1N H2SO4 / 1N HCl. TMB Substrate solution produces a soluble blue color with HRP, this reaction can be stopped with equal volume of 1N H2SO4 (sulphuric acid). The optical density of the resulting color solution can be read at 450 nm.

Direction for Use

• Reagent is clear to light yellow solution.
• Dilute required amount at 1:20 ratio in ultra pure water before use.
• Stop the reaction using
• 1N H2SO4
• Read optical density at 450 nm within 15 minutes after stopping the reaction

Precautions

• Once diluted, the reagent should be used immediately
• Do not store the diluted reagent
• Avoid direct exposure to light