Applications for Hank’s Balanced Salt Solution (HBSS) in cell culture include 66 cell washing, transferring tissue or cell samples, diluting cell populations for counting, and preparing reagents. The main function of a balanced salt solution is to supply cells with the water and essential inorganic ions they require to function while also maintaining the pH and osmotic balance. For the manufacture of reagents or transport media, formulations including calcium and magnesium are commonly utilized.