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Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody

Catalogue No BSAB-024S
Host Goat
Reactivity Rabbit
Applications WB, IHC
Size 1 MG
Price POR
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SKU: BSAB-024S Categories: ,
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Isotype IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Gamma Immunoglobins Heavy and Light chains
Conjugate HRP
Form Lyophilized
Concentration 1 mg/mL
Purification Affinity chromatography
Storage buffer PBS, pH 7.2, with 1% BSA
Contains 0.1% ProClin 150
Storage conditions Store at 4°C short term. For long term storage, store at -20°C,
Target IgG
Cross Adsorption Against bovine, goat, human, mouse and rat IgG and serum
Antibody Form Whole Antibody
Applications Tested Dilution
Western Blot (WB) 1:5,000-1:200,000
Immunohistochemistry (IHC) 1:500-1:20,000
ELISA (ELISA) 1:500-1:20,000

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, goat, human, mouse, or rat, IgG from bovine, goat, human, mouse or rat.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.