Description
GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.
| Catalogue No. | 0461KTB |
| Size | 96T |
| Applicable samples | Serum, Plasma, Animal Tissues, Cells, Bacteria |
| Kit components | • Assay Buffer
• Substrate • GR • H2O2 |
| Features & Benefits | • Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution. • Prepare mixed reagents for use, and place on ice. • The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed. • The reaction temperature has influence on the result. Keep the temperature at 25°C (for general species) or 37°C (for mammals). |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information. |
| Documents | Product manual with the material |
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