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General Estradiol , E2 ELISA Kit |
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Sensitivity |
1.33ng/mL |
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Range |
3.12-200ng/mL |
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Detection Method |
Competitive |
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Synonyms |
oestradiol,17β-estradiol |
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Sample Type |
For use with serum, plasma, culture media, or any biological fluids. |
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Intended Use |
Enzyme immunoassay for the quantitative detection of Canine Testosterone antigen in serum,plasma, culture media, or any biological fluid. |
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Storage |
2 - 8°C. |
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Principle |
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General E2, During the reaction, General E2 in the sample or standard competes with a fixed amount of biotin-labeled General E2 for sites on a pre-coated Monoclonal antibody specific to General E2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
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Function |
Estradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3). Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect. |
