Gel & PCR purification of DNA is a common technique for the isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields. Gel & PCR Clean Up Kit uses technology to recover DNA bands 100 bp – 20 kb from all grades of agarose gel and PCR products in yields exceeding 80%. The DNA band of interest is excised from the gel, dissolved in XP5 Buffer, and applied to a DNA spin column. Following a rapid wash step, DNA is eluted with Elution Buffer and is ready for other applications. The product is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions.
Benefits of Gel & PCR Clean Up Kit
- Speed – DNA recovery from agarose gel or PCR products< 10 minutes
- Reliability – with optimized buffers that guarantee pure DNA
- Safety – due to no organic extractions
- Quality – ensures that purified DNA will be suitable for any application
- High Concentrations -10-30ÎĽl elution volume