|
GAPDH Rabbit pAb |
|
Antibody type |
Polyclonal Antibody |
|
Gene Name |
GAPDH |
|
Gene ID |
2597 |
|
Alternative Name |
G3PD; GAPD; HEL-S-162eP |
|
Form |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Product Type |
Loading Control Antibody |
|
Recommended Dilutions |
WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200 IP: 1/20 FC: 1/100 |
|
Preservative |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Molecular Weight |
Calculated MW: 36 kDa; Observed MW: 36 kDa |
|
Isotype |
IgG |
|
Cellular localization |
Cytoplasm, Cytoskeleton, Membrane, Nucleus |
|
Purification |
Affinity Chromatography |
|
Immunogen |
A synthesized peptide derived from human GAPDH |
|
Storage/Stability |
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
|
Other Names |
aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685 |
|
Background |
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types. |
