ELISA Kit for Interleukin 12B (IL12B)

Catalogue No BSELK-035P
Size 96 T
Price $ 385
Range 7.8-500pg/mL
Sensitivity 3.5pg/mL
Species Sus Scrofa; Porcine (Pig)
Test Method Double-antibody Sandwich
Assay Length 3h
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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SKU: BSELK-035P Categories: ,

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 12B (IL12B). No significant cross-reactivity or interference between Interleukin 12B (IL12B) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 12B (IL12B) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 12B (IL12B) in samples.

Matrix Recovery Range(%) Average Recovery(%)
serum(n=5) 95-103 101
EDTA plasma(n=5) 88-102 95
heparin plasma(n=5) 80-96 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12B (IL12B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12B (IL12B) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 97-105% 85-105% 78-103%
EDTA plasma(n=5) 85-92% 86-101% 91-105% 97-105%
heparin plasma(n=5) 96-104% 88-98% 84-95% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Test Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve

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