DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications.
This DMEM is modified as follows:
With:Â High Glucose, L-glutamine, Phenol Red
Without:Â Sodium Pyruvate, HEPES
| Specifications | |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Glucose Concentration | 4500 mg/L |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco™ |
| Shelf Life | 12 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES |
| Unit Size | Each |
| Storage conditions | 2-8° C. Protect from light |
| Manfacturer | Thermo Fisher Scientific |
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
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