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3,3′-Diaminobenzidine

3,3′-Diaminobenzidine

CAS No: 91-95-2

Catalogue No BSCHM-041D
Mol. Formula (NH2)2C6H3C6H3(NH2)2
Mol. Weight 214.27
Price POR
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SKU: BSCHM-041D Categories: ,
3,3′-Diaminobenzidine, 97% (HPLC)
Synonyms 3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB
Grade HPLC
Assay 97% (HPLC)
Appearance (Form) Powder
Quality Segment 200
Melting Point 175-177 °C (lit.)
application(s) diagnostic assay manufacturing, hematology, histology
SMILES string NC1=C(N)C=CC(C2=CC(N)=C(N)C=C2)=C1
InChI 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key HSTOKWSFWGCZMH-UHFFFAOYSA-N
storage temp. room temp
Pack Size 1 GM | 5 GM | 10 GM | 25 GM

3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections.

Peroxidase substrate; reagent for spectrophotometric determination of selenium.
 

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.