| Deoxyribonuelease I，Dnase I | |
| Synonyms | DNase I Deoxyribonucleic Acid 5′-Oligonucleotide-hydrolytic enzyme |
| Appearance (Character) | White freeze-dried powder |
| Source | Bovine pancreas |
| Optimal pH value | 7-8 |
| Solubility | 0.15 M NaCl (5mg/ml) |
| Storage | Store at -20℃ |
| Enzyme activity | 1500 units/mg. There are differences in enzyme activitybetweendifferent batches, and the actual label should be used as the standard |
Deoxyribonuelease I Dnase I
| Catalogue No | BSCHM-035D |
| Mol. Formula | - |
| Mol. Weight | 31 kDa |
| Size | 1 MG | 25 MG | 100 MG |
deoxyribonucleic acid → dinucleotide-5’-phosphate + oligonucleotide-5’-phosphate
Inhibitor: EDTA, sodium dodecyl sulfate (SDS)
Activator: divalent metal ions, commonly used are Mg2+ and Mg2+ The best effect can be obtained by combining with Ca2+. 5 mmol/L Ca2+ can stabilize DNase I from protease digestion; 0.1mmol/L Ca2+ can reduce the inactivation rate by half.