Fungi, including yeasts and filamentous species or moulds are ubiquitously distributed in nature. Czapek-Dox Broth is used to grow bacteria and fungi which are capable of utilizing sodium nitrate as a sole source of nitrogen. This medi um is prepared according to the formula developed by Thom and Church, (1) having a defined chemical composition, which has been modified by the substitution of magnesium glycerophosphate for magnesium sulfate and potassium phosphate in the original formula (2, 3) as per Thomas and Raper.  Czapek-Dox media are useful in a variety of microbiological procedures, including soil microbiology and fungi and mildew resistance tests. Thom and Raper reported Czapek -Dox Broth and Czapek Solution Agar produce moderately vigorous growth of most saprophytic aspergilla And yield characteristic mycelia and conidia.

Sucrose serves as the sole source of carbon and sodium nitrate serves as the sole source of nitrogen. Dipotassium phosphate buffers the medium. Magnesium sulphate and ferrous sulfate are sources of cations, and potassium chloride contains essential ions.