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Cytochrome c Oxidase 1 Antibody |
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Description |
Rabbit Polyclonal Antibody to Cytochrome c Oxidase 1 |
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Application |
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200 |
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Clonality |
Polyclonal |
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Specificity |
Cytochrome c Oxidase 1 Antibody detects endogenous levels of total Cytochrome c Oxidase 1. |
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Conjugate |
Unconjugated |
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Purification |
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin. |
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Storage |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
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Immunogen |
A synthesized peptide derived from human Cytochrome c Oxidase 1, corresponding to a region within C-terminal amino acids. |
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Uniprot |
P00395(COX1_HUMAN) |
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Gene(ID) |
MT-CO1(4512) |
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Subcellular Location |
Mitochondrion inner membrane>Multi-pass membrane protein. |
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Function |
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. |
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Prediction |
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%) |
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Validated Image |
Western blot analysis of lysates from Rat brain, using Cytochrome c Oxidase 1 Ab at 1/1000 dilution
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