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Cox2 Recombinant Rabbit mAb |
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Description |
Rabbit Monoclonal Antibody to Cox2 |
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Application |
WB 1:1000, IHC: 1:100-1:1000, IF/ICC 1:50-1:200 |
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Clonality |
Monoclonal |
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Specificity |
Cox2 Recombinant Rabbit mAb detects endogenous levels of Cox2. |
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Conjugate |
Unconjugated. |
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Purification |
Affinity-chromatography |
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Storage |
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt |
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Immunogen |
A synthetic peptide from human Cox2 |
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Uniprot |
P35354(PGH2_HUMAN) |
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Gene(ID) |
PTGS2(5743) |
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Subcellular Location |
Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein. |
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Function |
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia (By similarity). |
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PTMs |
S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
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Description |
Prostaglandin-endoperoxide synthase (PTGS),also known as cyclooxygenase,is the key enzyme in prostaglandin biosynthesis,and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2,which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events,suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. |
