|
Cortisol ELISA Kit |
|
Sensitivity |
0.19ng/mL |
|
Range |
0.31-20ng/mL |
|
Uniprot ID |
N/A |
|
Alternative Names |
Cortisol |
|
Detection Method |
Competitive |
|
Sample Type |
Serum, Plasma, Other biological fluids,Sample volume: 50μL |
|
Standard |
20ng/mL |
|
Reaction Time |
1.5H |
|
Research Area |
Cardiovascular；Signaling transduction；Neuroscience；Cancer；Metabolism |
|
Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |
|
Cortisol ELISA Kit |
|
Sensitivity |
0.93 ng/mL |
|
Range |
3.13-200 ng/mL |
|
Product Name |
Cor(Cortisol) ELISA Kit |
|
Alternative Names |
Hydrocortisone |
|
Assay Type |
Competitive Inhibition |
|
Standard |
200 ng/mL |
|
Sample Type |
serum, plasma, urine and other biological fluids |
|
Assay Length |
2h |
|
Research Area |
Metabolic pathway, Endocrinology, Hormone metabolism |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |
