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Cofilin Antibody |
|
Description |
Rabbit Polyclonal Antibody To Cofilin |
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Cited expt |
WB |
|
Mol.Wt |
19kDa(Observed); 19kD(Calculated). |
|
Uniprot |
Q9Y281 | P23528 |
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Application |
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200 |
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Prediction |
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%) |
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Clonality |
Polyclonal |
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Specificity |
Cofilin Antibody detects endogenous levels of total Cofilin. |
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Conjugate |
Unconjugated. |
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Purification |
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin. |
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Storage |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
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Alternative Name |
CFL 2; CFL2; COF2_HUMAN; Cofilin 2 muscle; Cofilin; Cofilin muscle; Cofilin muscle isoform; Cofilin-2; Cofilin2; muscle isoform; NEM 7; NEM7; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18 |
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Gene(ID) |
CFL2(1073) | CFL1(1072) |
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Uniprot |
Q9Y281(COF2_HUMAN), P23528(COF1_HUMAN) |
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Immunogen |
A synthesized peptide derived from human Cofilin, corresponding to a region within the internal amino acids. |
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Description |
cofilin 1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. |
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PTMs |
The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. |
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Subcellular Location |
Nucleus matrix. Cytoplasm>Cytoskeleton |
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Family & Domains |
Belongs to the actin-binding proteins ADF family. |
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Tissue Specificity |
Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues. |
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Function |
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity). |
