Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cit protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cit. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cit in the samples is then determined by comparing the OD of the samples to the standard curve.
| Catalogue No. | ELK9241 |
| Size | 96T |
| Product Category | ELISA (Quantitative) |
| Reactivity | General |
| Sample | serum, plasma, urine and other biological fluids |
| Assay Type: | Competitive Inhibition |
| Assay Length | 2h |
| Sensitivity | 0.51 ng/mL |
| Range | 1.57-100 ng/mL |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information. |
| info@bio-string.com |
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