Applications Notes

Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.

Kit Components

• LiveDye
• NucleiDye
• Assay Buffer (10X)

Features & Benefits

• Suitable for mammalian cells staining.
• Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy.
• LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm).

Usage Notes

NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent.

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background

Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death.