| Cell Counting Kit-8 Assay Kit | |
| Description | Quantitation of viable cell number in proliferation and cytotoxicity assays |
| Detection Method | Colorimetric |
| Platform | Microplate reader 450 nm |
| Storage/Stability | Shipped and store at 4°C for 1 year. |
| Principle | WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible. |
Cell Counting Assay Kit-8
| Catalouge No | BSAK-024 |
| Reactivity | All |
| Applications | Functional Assay |
| Size | 100 | 500 Assays |
| Price | POR |
Cell Counting Kit-8 (CCK-8) provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4- nitrophenyl)-3-(4-nitrophenyl)-5-(2,4- disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator.
CCK-8 is a one-bottle solution, ready to use. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.