cDNA Kit is a comprehensive solution tailored for efficient and reliable cDNA synthesis. Engineered to meet the demands of diverse research applications, this kit combines a range of essential components to streamline the process of converting RNA into complementary DNA (cDNA).
At its core, the kit features flagship reverse transcriptase, a high-performance enzyme renowned for its robust and accurate reverse transcription capabilities. This enzyme is complemented by a meticulously formulated 5X reaction buffer, optimizing reaction conditions for maximum efficiency and yield.
To ensure versatility and adaptability, cDNA Kit includes a mix of random hexamers and oligo(dT) primers, catering to different RNA species and offering flexibility in target selection. Additionally, the presence of dithiothreitol (DTT) helps maintain enzyme stability and activity throughout the reaction, enhancing overall performance.
Furthermore, the kit incorporates a RNase inhibitor enzyme, safeguarding RNA integrity and preventing degradation during the reverse transcription process. Coupled with a dNTP mix and nuclease-free enzymes, researchers can trust in the purity and quality of their synthesized cDNA, free from contaminating nucleases.
Whether used for gene expression analysis, molecular cloning, or other downstream applications, cDNA Kit provides researchers with a reliable, efficient, and user-friendly solution for cDNA synthesis, empowering them to unlock new insights and discoveries in their fields of study.
Single Pack Contents
One pack is sufficient for 20µl X 50 reactions
- Reverse Transcriptase (200U/µL): 50 µL
- Rnase Inhibitor enzyme (40U/µL): 50µL
- Random Hexamers: 50µL
- OligodT: 50µL
- dNTP Mix: 50µL
- 5X Reaction Buffer: 200µL
- 100mM DTT: 50µL
- Nuclease Free Water: 1ml