|
Catalase (CAT) Activity Assay Kit |
|
Sensitivity |
0.27 U/mL |
|
Range |
0.27-155.4 U/mL |
|
Detection Method |
Colorimetric method |
|
Sample Type |
Serum,Plasma,Cell,Tissue |
|
Assay length |
1 h 10 min |
|
Sample Volume |
0.1-0.2 mL |
|
Precision |
inter-assay CV: 5.1% | intra-assay CV: 3.1% |
|
Storage |
This product can be stored at 2-8°C for 12 months with shading light. |
|
Detection Instrument |
Spectrophotometer (405 nm) |
|
Research Area |
Oxidative Stress |
|
Principle |
Catalase (CAT) can catalyze the decomposition of H2O2 into oxygen and water, and this reaction is quickly stopped upon the addition ofammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
