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Caspase-3 Activity Colorimetric Microplate Assay Kit |
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Applications |
Functional Assay |
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Description |
Detection and Quantification of Caspase-3 Activity. |
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Detection Method |
Colorimetric |
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Sample Types |
Tissue extracts, Cell lysate, Other biological fluids |
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Platform |
Microplate reader 405 nm |
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Storage/Stability |
Shipped and store at 4°C for 6 months |
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Principle |
Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and
pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3Activity Colorimetric Microplate Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage fromthe labeled substrate. The pNA light emission can be quantified using a microtiter plate reader at 405nm. The colorimetric intensity is proportional to the caspase-3 activity |
