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Carbonic Anhydrase 9 (CA9) ELISA Kit for Human |
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Sensitivity |
1.0pg/mL |
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Range |
250-5000pg/mL |
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Sample Type |
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate |
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Detection Method |
Competitive |
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Molecular Weight |
49,698 Da |
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Gene Name |
CA9 elisa kit |
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Storage |
Store all reagents at 2-8 degree C |
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Intended Uses |
This CA9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CA9. This ELISA kit for research use only, not for therapeutic applications! |
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Alternative Name |
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 |
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Protein Family |
Carbonic anhydrase |
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Principle |
CA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA9 antibody and an CA9-HRP conjugate. The assay sample and buffer are incubated together with CA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA9 concentration since CA9 from samples and CA9-HRP conjugate compete for the anti-CA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA9 from the sample, fewer sites are left to bind CA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA9 concentration in each sample is interpolated from this standard curve. |
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Validated Image |
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Manufacturer |
MyBioSource |
