Purpose

Our Canine Parvovirus Antibody (PV-Ab)ELISA Kit is to for the qualitative determination of PV-Ab in Canine serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to PV-Ab, make it
to solid-phase antigen .Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for PV-Ab is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain PV-Ab and HRP conjugated PV antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. The qualitative determination of PV-Ab is determined by comparing with the CUT OFF value.

Storage and Validity

• Storage: 2-8℃.
• Duration: 6 months