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Calcium (Ca) Colorimetric Assay Kit |
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Sensitivity |
0.07 mmol/L |
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Range |
0.07-1.2 mmol/L |
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Alternative Names |
Ca |
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Detection Method |
Colorimetric method |
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Sample Types |
Serum, Urine, Saliva, Other biological fluids |
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Platform |
Microplate reader (600-620 nm) |
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Research Area |
Plant Physiology Series, Inorganic Ions |
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Precision |
inter-assay CV: 8.5% | intra-assay CV: 4.7% |
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Sample Volume |
10 μL |
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Assay Time |
20 min |
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Storage |
2-8°C for 12 months |
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Principle |
Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715 nm and calcium content can be calculated by measuring the OD value at 610 nm |
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Validated Image |
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