Purpose
Our Bovine tumor necrosis factor α (TNF-α) ELISA kit is to assay TNF-α levels in Bovine serum, plasma, culture media or any biological fluid. Enzyme immunoassay for the quantitative detection of Bovine TNF-α antigen in serum, plasma, culture media, or any biological fluid.
| Bovine tumor necrosis factor α,TNF-α ELISA KIT - Details | |
| Wavelength | 450nm |
| Standard | 135pg/ml |
| Sample Volume | 10ul |
| Samples | serum, plasma, culture media, or any biological fluids |
| Detection Method | Sandwich-ELISA |
| Storage | 2-8℃ |
| Principle | |
| This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-α is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve. | |
Precision
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high levels of Bovine TNF-α were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Bovine TNF-α were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/mean X 100
- Intra-Assay: CV<10%
- Inter-Assay: CV<12%
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