Bovine T4(Thyroxine) ELISA Kit

Catalogue No BSFTEB-0140
Size 48T | 96T
Range 0.703-45ng/ml
Sensitivity 0.422ng/ml
Species Bovine
Contact Us
SKU: BSFTEB-0140 Categories: ,

This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with T4. T4 in the sample or standard competes with a fixed amount of biotin-labeled T4 for sites on a pre-coated antibody specific to T4. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of T4 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

Specifications

Product Name Bovine T4(Thyroxine) ELISA Kit
Alias T4 ELISA Kit, Thyroxine ELISA Kit
Sample Type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method Competitive ELISA, Coated with Antigen
Detection Wavelength OD450
Reaction Duration 2 hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with T4 , no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.). Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml) OD-1 OD-2 Average
0 2.748 2.828 2.788
0.703 1.671 1.719 1.695
1.406 1.032 1.062 1.047
2.812 0.646 0.664 0.655
5.625 0.438 0.45 0.444
11.25 0.325 0.335 0.33
22.5 0.258 0.266 0.262
45 0.172 0.178 0.175

Recovery

Add a certain amount of T4 into the sample. Calculate the recovery by comparing the measured value with the expected amount of T4 in the sample

Sample Type Recovery Range(%) Average(%)
serum(n=10) 89-105 98
EDTA plasma(n=10) 88-101 93
Heparin plasma(n=10) 89-96 93

Linearity

Dilute the sample with a certain amount of T4 at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 85-100% 91-101% 88-98%
EDTA plasma(n=10) 87-97% 83-98% 86-99%
Heparin plasma(n=10) 80-94% 86-99% 80-99%

Precision(%)

  • Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
  • Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/ml) 1.36 5.21 21.12 1.42 5.38 22.39
Standard deviation 0.07 0.27 0.96 0.09 0.28 1.09
CV(%) 5.23 5.1 4.56 6.02 5.24 4.89

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100

Reviews

There are no reviews yet.

Be the first to review “Bovine T4(Thyroxine) ELISA Kit”

Your email address will not be published. Required fields are marked *