Bovine SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit

Catalogue No BSFTEB-0164
Size 48T | 96T
Range 0.781-50ng/ml
Sensitivity 0.469ng/ml
Species Bovine
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SKU: BSFTEB-0164 Categories: ,

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SOD1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with SOD1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SOD1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Specification

Product Name Bovine SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
Alias Superoxide dismutase [Cu-Zn] ELISA Kit, Superoxide dismutase 1 ELISA Kit, hSod1 ELISA Kit, SOD1 ELISA Kit
Sample Type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method Sandwich ELISA, Double Antibody
Detection Wavelength OD450
Reaction Duration 4 hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with SOD1 , no obvious cross reaction with other analogues.
UniProt ID P00442

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.). Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml) OD-1 OD-2 Average
0 0.112 0.116 0.114
0.781 0.235 0.241 0.238
1.562 0.394 0.406 0.4
3.125 0.572 0.588 0.58
6.25 0.795 0.819 0.807
12.5 1.046 1.076 1.061
25 1.584 1.63 1.607
50 2.278 2.344 2.311

Recovery

Add a certain amount of SOD1 into the sample. Calculate the recovery by comparing the measured value with the expected amount of SOD1 in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 90-102 96
EDTA plasma(n=10) 89-100 97
Heparin plasma(n=10) 86-103 91

Linearity

Dilute the sample with a certain amount of SOD1 at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 85-103% 85-105% 92-105%
EDTA plasma(n=10) 86-98% 85-99% 82-100%
Heparin plasma(n=10) 82-98% 82-98% 85-96%

Precision(%)

  • Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
  • Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/ml) 1.65 5.81 23.68 1.5 6.2 25.21
Standard deviation 0.09 0.27 1.01 0.08 0.31 1.49
CV(%) 5.26 4.68 4.27 5.39 4.95 5.91

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100

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