Our Bovine Interleukin10(IL-10) ELISA kit is to assay IL-10 levels in Bovine serum, plasma, culture media or any biological fluid. Enzyme immunoassay for the quantitative detection of Bovine IL-10 antigen in serum, plasma, culture media, or any biological fluid.
| Bovine Interleukin10 (IL-10) ELISA KIT | |
| Sensitivity | 0.5 pg/ml |
| Range | 3 pg/ml - 200 pg/ml |
| Wavelength | 450nm |
| Standard | 270 pg/ml |
| Sample Volume | 10ul |
| Samples | serum, plasma, culture media, or any biological fluids |
| Detection Method | Sandwich-ELISA |
| Storage | 2-8℃ |
| Principle | This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-10 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-10 and HRP conjugated IL-10 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-10. You can calculate the concentration of IL-10 in the samples by comparing the OD of the samples to the standard curve. |