Purpose
Enzyme immunoassay for the quantitative detection of Bovine IGF-1 antigen in serum, plasma, culture media, or any biological fluid.
| Bovine Insulin-like growth factors 1 (IGF-1) - Details | |
| Wavelength | 450nm |
| Standard | 18ng/ml |
| Sample Volume | 10ul |
| Samples | serum, plasma, culture media, or any biological fluids |
| Detection Method | Sandwich-ELISA |
| Storage | 2-8℃ |
| Principle | |
| The quantitative detection of Bovine IGF-1 antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Bovine IGF-1. The corresponding antigen in the sample or standards binds the immobilized antibody after incubation. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate specific to IGF-1 is added, which binds the captured antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, the unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve | |
Precision
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high levels of Bovine IGF-1 were tested 20 times on one plate, respectively
- Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Bovine IGF-1 were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/mean X 100
- Intra-Assay: CV<10%
- Inter-Assay: CV<12%
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