Bovine IFN-γ(Interferon Gamma) ELISA Kit

Bovine IFN-γ(Interferon Gamma) ELISA Kit

Bovine IFN-γ(Interferon Gamma) ELISA Kit

Catalogue No.	BSEB1000
Size	48T \| 96T
Detection Range	15.625-1000pg/ml
Sensitivity	9.375pg/ml
Test Method	Sandwich ELISA, Double Antibody
Sample Type	Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Categories: BOVINE- ELISA KIT, Elisa Kits

Description

Alias	Interferon gamma ELISA Kit, BoIFNG ELISA Kit, IFN-gamma ELISA Kit, IFNG ELISA Kit
Storage	2-8°C(Sealed), Don’t cryopreserve.
Specificity	Specifically binds with IFN-γ , no obvious cross reaction with other analogues.
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IFN-γ antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IFN-γ bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IFN-γ in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

ELISA Kit Components

Kit Components	Item	Size(48T)	Size(96T)	Storage Condition for Opened Kit
E001	ELISA Microplate(Dismountable)	8×6	8×12	Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002	Lyophilized Standard	1vial	2vial	Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003	Biotin-labeled Antibody(Concentrated, 100X)	60ul	120ul	2-8°C (Avoid Direct Light)
E034	HRP-Streptavidin Conjugate(SABC, 100X)	60ul	120ul
E024	TMB Substrate	5ml	10ml
E039	Sample Dilution Buffer	10ml	20ml	2-8°C
E040	Antibody Dilution Buffer	5ml	10ml
E049	SABC Dilution Buffer	5ml	10ml
E026	Stop Solution	5ml	10ml
E038	Wash Buffer(Concentrated, 25X)	15ml	30ml
E006	Plate Sealer	3 pieces	5 pieces
E007	Product Description	1 copy	1 copy

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