| Sensitivity | 0.04ng/ml |
| Range | 0.16ng/ml-10ng/ml |
| Sample Volume | 10ul |
| Wavelength | 450nm |
| Standard | 13.5ng/ml |
| Principle | This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to CK-MB. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CK-MB is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CK-MB and HRP conjugated CK-MB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CK-MB. You can calculate the concentration of CK-MB in the samples by comparing the OD of the samples to the standard curve. |
Bovine Creatine Kinase MB Isoenzyme Elisa Kit
| Catalogue No | BSSL-0269Bo |
| Species | Bovine |
| Size | 48T | 96T |
| Price | POR |
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high levels of Bovine CK-MB were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Bovine CK-MB were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/mean X 100
- Intra-Assay: CV<10%
- Inter-Assay: CV<12%
Our Bovine Creatine Kinase MB isoenzyme (CK-MB) ELISA kit is to assay CK-MB levels in Bovine serum, plasma, culture media or any biological fluid.