Purpose
Our Bovine Cortisol ELISA kit is to assay Cortisol levels in Bovine serum, plasma, culture media or any biological fluid. Enzyme immunoassay for the quantitative detection of Bovine Cortisol antigen in serum, plasma, culture media, or any biological fluid.
| Bovine Cortisol ELISA kit - Details | |
| Wavelength | 450nm |
| Standard | 135 ng/ml |
| Sample Volume | 10ul |
| Detection Method | Sandwich |
| Storage | 2-8°C |
| Principle | |
| This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to Cortisol. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Cortisol is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Cortisol and HRP conjugated Cortisol antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Cortisol. You can calculate the concentration of Cortisol in the samples by comparing the OD of the samples to the standard curve. | |
Precision
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high levels of Bovine Cortisol were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Bovine Cortisol were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/mean X 100
- Intra-Assay: CV<10%
- Inter-Assay: CV<12%
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