This column is designed for workflows where nucleic acids are present in low abundance, particularly in viral and cell-free sample preparation.

Compared with general-purpose columns, the membrane structure is optimized to improve capture efficiency under dilute conditions, which is often required in plasma, serum, or other low biomass samples.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.