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BioString Universal DNA Kit

BioString Universal DNA Kit

Catalogue No BS-DNA-047
Product Name BioStringTM Universal DNA Kit
Size 50 Preps | 250 Preps
Price POR
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Features Specifications
Main Functions Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications PCR, qPCR, southern bolt and virus detection, etc
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume ≥20μl
Time per run 30 - 90 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg
Kit Contents
Contents BS-DNA-047 BS-DNA-047
Purification Times 50 250 Preps
HyPure DNA Mini Columns I 50 250
2ml Collection Tubes 100 500
Buffer ATL 30 ML 150 ML
Buffer AL 30 ML 150 ML
Buffer GW1 22 ML 88 ML
Buffer GW2 12 ML 50 ML
Proteinase K 24 MG 120 MG
Protease Dissolve Buffer 1.8 ML 10 ML
Buffer AE 15 ML 60 ML

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Validation experiments using multiple biological sample types demonstrated stable genomic DNA purification with purity ratios typically A260/280 around 1.7–1.9, indicating high-quality genomic DNA suitable for downstream molecular analysis workflows.

Extraction experiments across different tissue samples showed reproducible DNA yields with variation between replicate extractions typically below 10%, demonstrating stable purification performance across multiple sample types.

Purified genomic DNA produced clear high-molecular-weight bands in agarose gel electrophoresis and supported successful PCR amplification of target gene fragments, confirming compatibility with common molecular biology applications such as PCR amplification and genotyping analysis.

Comparative testing against commonly used commercial genomic DNA purification systems showed comparable DNA purity and recovery performance, supporting reliable DNA preparation for routine laboratory workflows.

Genomic DNA purified using the BioStringTM Universal DNA Kit is suitable for a wide range of molecular biology and molecular diagnostics research workflows, including:

  • PCR amplification
  • Genotyping analysis
  • HLA typing studies
  • Molecular diagnostics research
  • Population genetics studies
  • Sample preparation for sequencing workflows
  • Biobanking sample processing