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BioString PureSol Blood DNA Kit

DNA-RNA- Protein-Kit

BioString PureSol Blood DNA Kit

Catalouge No BS-DNA-048
Product Name BioStringTM PureSol Blood DNA Kit
Size 50 ML | 300 ML | 1000 ML
Price POR
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SKU: BS-DNA-048 Categories: ,
Features Specifications
Main Functions Isolation total DNA from whole blood using economic salt out method
Applications PCR, enzyme digestion, Southern hybridization,etc.
Purification Technology Salting out method
Process Method Manual (centrifugation or vacuum)
Sample Type Animal tissue
Sample Amount Unlimited
Elution Volume ≥100μl
Time Per Run Variation with sample amount
Kit Contents
Contents BS-DNA-048 BS-DNA-048 BS-DNA-048
Purification Volumes 50 ml 300 ml 1000 ml
10 x Buffer RBC 20 ML 100 ML 3 x 100 ML
Buffer WTL 60 ML 350 ML 1000 ML
Buffer PPS 20 ML 120 ML 350 ML
RNase Solution 300 ML 1.8 ML 6 ML
Buffer TE 10 ML 30 ML 120 ML

BioStringTM PureSol Blood DNA Kit provides a salt precipitation workflow for purification of high-molecular-weight genomic DNA from whole blood and related biological fluids. The protocol uses detergent-mediated cell lysis followed by protein precipitation and alcohol-based DNA recovery, producing genomic DNA fragments that can exceed 200 kb in size.

The scalable purification workflow allows preparation of genomic DNA suitable for PCR analysis, sequencing preparation, or DNA archiving from routine blood samples.

Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

BioStringTM PureSol Blood DNA Kit was validated using multiple blood sample types, including 1 mL human anticoagulated blood, 10 mL human anticoagulated blood, long-term stored anticoagulated blood, 5 mL porcine anticoagulated blood and pre-homogenized coagulated porcine blood. The extracted DNA showed A260/280 values of approximately 1.8–2.0, supporting reliable protein removal and DNA purity in large-volume blood DNA extraction workflows.

DNA yield increased with blood input volume and sample type. In the tested conditions, 1 mL human blood produced approximately 39 µg DNA, 10 mL human blood produced more than 300 µg DNA, and 5 mL porcine blood produced approximately 398–412 µg DNA. Stored and coagulated blood samples also produced recoverable DNA when appropriate pretreatment was used.

Agarose gel electrophoresis showed high-molecular-weight genomic DNA bands above 23 kb without obvious smearing, indicating good DNA integrity. PCR validation using human PP1 primers and porcine-specific primers further confirmed that the purified DNA was suitable for downstream amplification workflows.

  • Safe - without phenol chloroform extraction
  • Environment friendly - the reagents used are safe, non-toxic and without pollution
  • High molecular weight - the molecular weight of genomic DNA is about 50-150kb
  • High purity - the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
  • Unlimited sample size - solution type operation, sample volume can be adjusted at will
  • Cost performance - the most economical nucleic acid extraction technology