Ensure maximum protein integrity and yield with our advanced BioString HM Protease Inhibitor. During cell lysis, natural protease control mechanisms are disrupted, leading to unwanted proteolysis, protein degradation, and reduced recovery. Our high-performance protease inhibitor effectively prevents protein loss by blocking a broad range of proteases, including pepsin, trypsin, chymotrypsin, pronase E, bromelain, papain, cathepsin B, kallikrein-3, subtilisin, elastase, lysozyme, thrombin, thermolysin, and collagenase. Whether for protein purification, cell extraction, or protein isolation protocols, BioString HM delivers reliable and consistent protection for all your valuable samples.

Specificity

This product is a specifically synthesized protease inhibitor which is effective for serine proteases, aspartic proteases and cysteine endopeptidases.

Recommended Buffer Conditions

• Lysis buffer
• Radioimmunoprecipitation assay buffer (RIPA) lysis buffer composition (300mM NaCl, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS, 50mM Tris pH 8.0.
• NP-40 tissue lysis bufferNP-40 buffer composition (50mM Tris-Cl pH 7.5, 150mM NaCl, 0.5% NP-40, 1mM Na-pyrophosphate, 5mM Na-orthovanadate, 1mM Benzamidine, 10mM Sodium fluoride)
• Phenol buffer for plant protein extraction Phenol buffer composition (0.7M Sucrose, 0.5 M Tris, 30mM HCl, 50mM EDTA, 0.1M KCl)
• Tris, NaCl Buffer
• Water