The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the BioString^TM DNA/RNA/Protein Kit Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

The Kit isolates total RNA from up to 10 ⁷ cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.

• High quality - high purity total RNA / DNA can be directly used in a variety of downstream applications
• Fast - column method can complete the extraction of several samples in 30 minutes
• Safe - no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

BioString^TM DNA/RNA/Protein Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.