Mini Column II extends the standard column format by increasing membrane layers to improve binding capacity. This makes it more suitable for plasmid preparation and workflows where DNA input or expected yield is higher than routine levels.

In comparison to the basic mini format, it provides better tolerance to sample loading variability while maintaining stable purification performance.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.