The Micro Column is positioned between nano-scale recovery and routine purification. It uses a multi-layer glass fiber structure to increase binding capacity while maintaining compatibility with small sample volumes.

In laboratory workflows, this format is commonly selected when sample input is still limited but higher yield stability is required compared with nano columns. It provides a more balanced option for viral nucleic acid preparation and micro-scale DNA extraction tasks.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.