This column variant is designed for workflows where higher centrifugation speed is available. It allows faster processing of plasmid purification steps compared with standard maxi formats.

The column structure is adapted to maintain stability under higher centrifugal force while preserving nucleic acid binding performance.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.